Perfusion
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Revision as of 01:28, 27 November 2013 by Kristin Engberg (Talk | contribs)
Transcardial perfusion of the animal using the hydrogel solution in place of normal fixative is recommended, but not essential. By adding the acrylamide monomers into the tissue at the same time as paraformaldehyde, it is easier for the acrylamide to attach to the biomolecules.