Chemical Reagents
From CLARITY Wiki
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==Hydrogel solution reagents== |
==Hydrogel solution reagents== |
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===American suppliers=== |
===American suppliers=== |
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| − | ===European suppliers=== |
+ | ==Clearing solution reagents== |
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| − | | 40% acrylamide solution |
+ | | Boric acid |
| − | | Hydrogel monomer solution, 500 mL |
+ | | pH buffer |
| − | | |
+ | | Sigma (B7901) |
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| − | | 2% bis-acrylamide solution |
+ | | NaOH pellets |
| − | | Chemical crosslinker, 500 mL |
+ | | Adjust solution pH to 8.5 |
| − | | |
+ | | EMD (SX0590-3) |
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| − | | Formaldehyde |
+ | | SDS (powder or aqueous solution) |
| − | | Biomolecule crosslinker, 16% paraformaldehyde aqueous solution |
+ | | Lipid-clearing detergent |
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+ | | Sigma (L3771); Amresco (0837) |
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| − | | VA-044 photoinitiator |
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| − | | Non-nitrile azo thermal initiator with low decomposition temperature |
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| − | |- |
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| − | | 10X PBS |
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| − | | Solution buffer, 500 mL |
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Latest revision as of 01:21, 15 December 2016
Listed below are the chemicals necessary to make both the hydrogel and clearing solutions as well as suggested suppliers.
Contents |
[edit] Hydrogel solution reagents
[edit] American suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| 40% acrylamide solution | Hydrogel monomer solution, 500 mL | Bio-Rad (161-0140) |
| 2% bis-acrylamide solution | Chemical crosslinker, 500 mL | Bio-Rad (161-0142) |
| Formaldehyde | Biomolecule crosslinker, 16% paraformaldehyde aqueous solution | Electron Microscopy Sciences (15710-S) |
| VA-044 photoinitiator | Non-nitrile azo thermal initiator with low decomposition temperature | Wako (VA-044) |
| 10X PBS | Solution buffer, 500 mL | Invitrogen GIBCO (10010) |
[edit] Clearing solution reagents
[edit] American suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Boric acid | pH buffer | Sigma (B7901) |
| NaOH pellets | Adjust solution pH to 8.5 | EMD (SX0590-3) |
| SDS (powder or aqueous solution) | Lipid-clearing detergent | Sigma (L3771); Amresco (0837) |