Troubleshooting

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==ETC Set-up==
 
==ETC Set-up==
 
===Sample turns brown after running ETC===
 
===Sample turns brown after running ETC===
  +
The tissue sample should not turn brown during ETC. This is an indication of damage to/melting of the tissue. Throughout ETC, the tissue should instead remain a whitish to pale yellow color. Most likely, the damage to the sample is from the temperature inside the chamber getting too hot. This can be fixed by increasing the flow rate of the circulating clearing solution and by lowering either the set temperature or voltage (or both). For more information on ETC settings, see [[Electrophoretic Tissue Clearing|ETC set-up]].

Revision as of 00:59, 3 December 2013

CLARITY is a multi-step process, and sometimes it can be a little tricky to get all the steps working correctly, especially when starting out. Here are some common problems and concerns that may be encountered along with suggestions for troubleshooting.

Contents

Hydrogel Polymerization

After 3 hours of polymerization at 37°C, the solution is still liquid (no gel is formed)

Trouble removing the gel from the tissue sample without causing damage

ETC Set-up

Sample turns brown after running ETC

The tissue sample should not turn brown during ETC. This is an indication of damage to/melting of the tissue. Throughout ETC, the tissue should instead remain a whitish to pale yellow color. Most likely, the damage to the sample is from the temperature inside the chamber getting too hot. This can be fixed by increasing the flow rate of the circulating clearing solution and by lowering either the set temperature or voltage (or both). For more information on ETC settings, see ETC set-up.