Supplies

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Listed on this page are recommedations for supplies used in the hydrogel embedding, ETC, and sample mounting steps of CLARITY. Most of these supplies can easily be alternated for similar supplies that perform the same task.

Contents

Degassing supplies

American suppliers

Supply Purpose/Description Manufacturer (Part #)
Vacuum pump Degassing oxygen from container before hydrogel polymerization Buchi (V-700)
Desiccation canister Canister with two outlets for nitrogen/vacuum hook-up McMaster-Carr (2204K7)
Desiccation cartridge Cartridge for canister to hold samples McMaster-Carr (2204K71)
Nitrogen tank Replaces oxygen during degassing any

ETC set-up supplies

American suppliers

Supply Purpose/Description Manufacturer (Part #)
Temperature controlled water circulator Circulates and heats/cools clearing solution during ETC Lauda-Brinkmann (ECO RE 415S)
Buffer filter Filter and canister for collecting large particle contaminants from clearing solution McMaster-Carr (4448K35)
Replacement filter cartridge Replacement filters for clearing solution filtration (replace every few ETC runs) McMaster-Carr (4422K61)
BioRad HC PowerPac system Power supply for high-current applications, 100-120/220-240 V Bio-Rad (164-5052)
PowerPac system adaptor 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies Bio-Rad (164-5064)
Patch cord for banana plugs to alligator clips, 12" Cord to connect power supply to Pt wire of the ETC chamber McMaster-Carr (6927K42)
Clear 1/4" tubing Plastic tubing for connecting ETC components with barbed fittings McMaster-Carr
Clear polyurethane tubing Plastic tubing for connecting ETC components with push-to-connect fittings McMaster-Carr (5648K71)
Chemical resistant polypropylene stopcock Stopcock with barbed fittings for connecting 1/4" tubing McMaster-Carr (48285K24)
Polypropylene push-to-connect ball valve Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow) McMaster-Carr (4379K62)
Single barbed tube fitting, 9/16" hex head for 1/4" tubing Barbed fitting for connecting tubing to buffer filter McMaster-Carr (5463K247)
Teflon tape Wrap for sealing screwed fittings McMaster-Carr (7562A17)

ETC chamber supplies

American suppliers

Supply Purpose/Description Manufacturer (Part #)
Plastic chamber, 60 mL ETC container Nalgene (2118-0002)
Platinum wire, 0.5 mm diameter, 40-50 cm Wire for ETC electrodes Sigma Aldrich (267201-2G)
Single barbed tube fitting, 7/16" hex head for 1/4" tubing Barbed fitting for chamber inlet and outlet McMaster-Carr (5463K245)
Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size Push-to-connect fitting for chamber inlet and outlet McMaster-Carr (9087K13)
Tube to tube coupling for 3/32" x 1/16" tubing Electrode portal for connection to power supply McMaster-Carr (5117K51)
3M Duo Adhesive dispenser Epoxy adhesive dispenser McMaster-Carr (7467A43)
3M Duo Adhesive cartridges 50 mL epoxy adhesive cartridges McMaster-Carr (7467A17)
3M Duo Adhesive mixing applicators Cartridge tip to mix duo adhesive prior to application McMaster-Carr (7467A12)
Cell strainer Tissue sample holder inside chamber BD Falcon (352340)
Polyethylene mesh sleeve, 1/4"-1" diameter Mesh to place around tissue sample within holder to prevent sample movement/tumbling McMaster-Carr (5969K21/5969K22)
Chemical resistant polypropylene mesh, 6x6, 0.1323" opening Mesh to place between sample holder and electrodes McMaster-Carr (9275T38)
Electronic pliers Pliers for shaping Pt wires into electrodes Xcelite (378M)
Aluminum foil tape Tape for lightproofing sample chamber E&K Scientific (T592100)

European suppliers

Supply Purpose/Description Manufacturer (Part #)
Plastic chamber, 60 mL ETC container
Platinum wire, 0.5 mm diameter, 40-50 cm Wire for ETC electrodes
Single barbed tube fitting, 7/16" hex head for 1/4" tubing Barbed fitting for chamber inlet and outlet
Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size Push-to-connect fitting for chamber inlet and outlet
Tube to tube coupling for 3/32" x 1/16" tubing Electrode portal for connection to power supply
3M Duo Adhesive dispenser Epoxy adhesive dispenser
3M Duo Adhesive cartridges 50 mL epoxy adhesive cartridges
3M Duo Adhesive mixing applicators Cartridge tip to mix duo adhesive prior to application
Cell strainer Tissue sample holder inside chamber
Polyethylene mesh sleeve, 1/4"-1" diameter Mesh to place around tissue sample within holder to prevent sample movement/tumbling
Chemical resistant polypropylene mesh, 6x6, 0.1323" opening Mesh to place between sample holder and electrodes
Electronic pliers Pliers for shaping Pt wires into electrodes
Aluminum foil tape Tape for lightproofing sample chamber

Imaging chamber supplies

American suppliers

Supply Purpose/Description Manufacturer (Part #)
WillCo-dish PELCO black wall glass bottom dish Ted Pella (14032)
BluTack Sticky tack for holding glass dishes/plates together to form chamber Bostik
Kwik-Sil Quick-curing silicone elastomer to seal imaging chamber World Precision Instruments (KWIK-SIL)
FocusClear Refractive index matching immersion medium CelExplorer Labs

European suppliers

Supply Purpose/Description Manufacturer (Part #)
WillCo-dish PELCO black wall glass bottom dish
BluTack Sticky tack for holding glass dishes/plates together to form chamber
Kwik-Sil Quick-curing silicone elastomer to seal imaging chamber
FocusClear Refractive index matching immersion medium CelExplorer Labs

Other supplies

Supply Purpose/Description Manufacturer (Part #)
1 mm brain matrix Brain mold with inserts for razor blades to make uniform coronal sections after embedding ASI Instruments (RBM-2000C)
ThermaSeal Adhesive film for well plates to prevent evaporation during staining E&K Scientific (T796100)