Supplies
From CLARITY Wiki
(Difference between revisions)
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| Tape for lightproofing sample chamber |
| Tape for lightproofing sample chamber |
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+ | |} |
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+ | ==Imaging chamber supplies== |
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+ | ===American suppliers=== |
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+ | {| class="wikitable" |
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+ | |- |
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+ | ! Supply |
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+ | ! Purpose/Description |
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+ | ! Manufacturer (Part #) |
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+ | |- |
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+ | | WillCo-dish |
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+ | | PELCO black wall glass bottom dish |
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+ | | Ted Pella (14032) |
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+ | |- |
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+ | | BluTack |
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+ | | Sticky tack for holding glass dishes/plates together to form chamber |
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+ | | Bostik |
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+ | |- |
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+ | | Kwik-Sil |
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+ | | Quick-curing silicone elastomer to seal imaging chamber |
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+ | | World Precision Instruments (KWIK-SIL) |
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+ | |- |
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+ | | FocusClear |
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+ | | Refractive index matching immersion medium |
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+ | | CelExplorer Labs |
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+ | |} |
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+ | ===European suppliers=== |
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+ | {| class="wikitable" |
||
+ | |- |
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+ | ! Supply |
||
+ | ! Purpose/Description |
||
+ | ! Manufacturer (Part #) |
||
+ | |- |
||
+ | | WillCo-dish |
||
+ | | PELCO black wall glass bottom dish |
||
+ | | |
||
+ | |- |
||
+ | | BluTack |
||
+ | | Sticky tack for holding glass dishes/plates together to form chamber |
||
+ | | |
||
+ | |- |
||
+ | | Kwik-Sil |
||
+ | | Quick-curing silicone elastomer to seal imaging chamber |
||
+ | | |
||
+ | |- |
||
+ | | FocusClear |
||
+ | | Refractive index matching immersion medium |
||
+ | | CelExplorer Labs |
||
+ | |} |
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+ | ==Other supplies== |
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+ | {| class="wikitable" |
||
+ | |- |
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+ | ! Supply |
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+ | ! Purpose/Description |
||
+ | ! Manufacturer (Part #) |
||
+ | |- |
||
+ | | 1 mm brain matrix |
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+ | | Brain mold with inserts for razor blades to make uniform coronal sections after embedding |
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+ | | ASI Instruments (RBM-2000C) |
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+ | |- |
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+ | | ThermaSeal |
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+ | | Adhesive film for well plates to prevent evaporation during staining |
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+ | | E&K Scientific (T796100) |
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|} |
|} |
Revision as of 06:34, 24 January 2014
Contents |
Degassing supplies
American suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Vacuum pump | Degassing oxygen from container before hydrogel polymerization | Buchi (V-700) |
Desiccation canister | Canister with two outlets for nitrogen/vacuum hook-up | McMaster-Carr (2204K7) |
Desiccation cartridge | Cartridge for canister to hold samples | McMaster-Carr (2204K71) |
Nitrogen tank | Replaces oxygen during degassing | any |
European suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Vacuum pump | Degassing oxygen from container before hydrogel polymerization | |
Desiccation canister | Canister with two outlets for nitrogen/vacuum hook-up | |
Desiccation cartridge | Cartridge for canister to hold samples | |
Nitrogen tank | Replaces oxygen during degassing | any |
ETC set-up supplies
American suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Temperature controlled water circulator | Circulates and heats/cools clearing solution during ETC | Lauda-Brinkmann (ECO RE 415S) |
Buffer filter | Filter and canister for collecting large particle contaminants from clearing solution | McMaster-Carr (4448K35) |
Replacement filter cartridge | Replacement filters for clearing solution filtration (replace every few ETC runs) | McMaster-Carr (4422K61) |
BioRad HC PowerPac system | Power supply for high-current applications, 100-120/220-240 V | Bio-Rad (164-5052) |
PowerPac system adaptor | 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies | Bio-Rad (164-5064) |
Patch cord for banana plugs to alligator clips, 12" | Cord to connect power supply to Pt wire of the ETC chamber | McMaster-Carr (6927K42) |
Clear 1/4" tubing | Plastic tubing for connecting ETC components with barbed fittings | McMaster-Carr |
Clear polyurethane tubing | Plastic tubing for connecting ETC components with push-to-connect fittings | McMaster-Carr (5648K71) |
Chemical resistant polypropylene stopcock | Stopcock with barbed fittings for connecting 1/4" tubing | McMaster-Carr (48285K24) |
Polypropylene push-to-connect ball valve | Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow) | McMaster-Carr (4379K62) |
Single barbed tube fitting, 9/16" hex head for 1/4" tubing | Barbed fitting for connecting tubing to buffer filter | McMaster-Carr (5463K247) |
Teflon tape | Wrap for sealing screwed fittings | McMaster-Carr (7562A17) |
European suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Temperature controlled water circulator | Circulates and heats/cools clearing solution during ETC | |
Buffer filter | Filter and canister for collecting large particle contaminants from clearing solution | |
Replacement filter cartridge | Replacement filters for clearing solution filtration (replace every few ETC runs) | |
BioRad HC PowerPac system | Power supply for high-current applications, 100-120/220-240 V | |
PowerPac system adaptor | 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies | |
Patch cord for banana plugs to alligator clips, 12" | Cord to connect power supply to Pt wire of the ETC chamber | |
Clear 1/4" tubing | Plastic tubing for connecting ETC components with barbed fittings | |
Clear polyurethane tubing | Plastic tubing for connecting ETC components with push-to-connect fittings | |
Chemical resistant polypropylene stopcock | Stopcock with barbed fittings for connecting 1/4" tubing | |
Polypropylene push-to-connect ball valve | Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow) | |
Single barbed tube fitting, 9/16" hex head for 1/4" tubing | Barbed fitting for connecting tubing to buffer filter | |
Teflon tape | Wrap for sealing screwed fittings |
ETC chamber supplies
American suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Plastic chamber, 60 mL | ETC container | Nalgene (2118-0002) |
Platinum wire, 0.5 mm diameter, 40-50 cm | Wire for ETC electrodes | Sigma Aldrich (267201-2G) |
Single barbed tube fitting, 7/16" hex head for 1/4" tubing | Barbed fitting for chamber inlet and outlet | McMaster-Carr (5463K245) |
Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size | Push-to-connect fitting for chamber inlet and outlet | McMaster-Carr (9087K13) |
Tube to tube coupling for 3/32" x 1/16" tubing | Electrode portal for connection to power supply | McMaster-Carr (5117K51) |
3M Duo Adhesive dispenser | Epoxy adhesive dispenser | McMaster-Carr (7467A43) |
3M Duo Adhesive cartridges | 50 mL epoxy adhesive cartridges | McMaster-Carr (7467A17) |
3M Duo Adhesive mixing applicators | Cartridge tip to mix duo adhesive prior to application | McMaster-Carr (7467A12) |
Cell strainer | Tissue sample holder inside chamber | BD Falcon (352340) |
Polyethylene mesh sleeve, 1/4"-1" diameter | Mesh to place around tissue sample within holder to prevent sample movement/tumbling | McMaster-Carr (5969K21/5969K22) |
Chemical resistant polypropylene mesh, 6x6, 0.1323" opening | Mesh to place between sample holder and electrodes | McMaster-Carr (9275T38) |
Electronic pliers | Pliers for shaping Pt wires into electrodes | Xcelite (378M) |
Aluminum foil tape | Tape for lightproofing sample chamber | E&K Scientific (T592100) |
European suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
Plastic chamber, 60 mL | ETC container | |
Platinum wire, 0.5 mm diameter, 40-50 cm | Wire for ETC electrodes | |
Single barbed tube fitting, 7/16" hex head for 1/4" tubing | Barbed fitting for chamber inlet and outlet | |
Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size | Push-to-connect fitting for chamber inlet and outlet | |
Tube to tube coupling for 3/32" x 1/16" tubing | Electrode portal for connection to power supply | |
3M Duo Adhesive dispenser | Epoxy adhesive dispenser | |
3M Duo Adhesive cartridges | 50 mL epoxy adhesive cartridges | |
3M Duo Adhesive mixing applicators | Cartridge tip to mix duo adhesive prior to application | |
Cell strainer | Tissue sample holder inside chamber | |
Polyethylene mesh sleeve, 1/4"-1" diameter | Mesh to place around tissue sample within holder to prevent sample movement/tumbling | |
Chemical resistant polypropylene mesh, 6x6, 0.1323" opening | Mesh to place between sample holder and electrodes | |
Electronic pliers | Pliers for shaping Pt wires into electrodes | |
Aluminum foil tape | Tape for lightproofing sample chamber |
Imaging chamber supplies
American suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
WillCo-dish | PELCO black wall glass bottom dish | Ted Pella (14032) |
BluTack | Sticky tack for holding glass dishes/plates together to form chamber | Bostik |
Kwik-Sil | Quick-curing silicone elastomer to seal imaging chamber | World Precision Instruments (KWIK-SIL) |
FocusClear | Refractive index matching immersion medium | CelExplorer Labs |
European suppliers
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
WillCo-dish | PELCO black wall glass bottom dish | |
BluTack | Sticky tack for holding glass dishes/plates together to form chamber | |
Kwik-Sil | Quick-curing silicone elastomer to seal imaging chamber | |
FocusClear | Refractive index matching immersion medium | CelExplorer Labs |
Other supplies
Supply | Purpose/Description | Manufacturer (Part #) |
---|---|---|
1 mm brain matrix | Brain mold with inserts for razor blades to make uniform coronal sections after embedding | ASI Instruments (RBM-2000C) |
ThermaSeal | Adhesive film for well plates to prevent evaporation during staining | E&K Scientific (T796100) |