Supplies

From CLARITY Wiki

(Difference between revisions)
Jump to: navigation, search
(American suppliers)
 
(10 intermediate revisions by one user not shown)
Line 1: Line 1:
  +
Listed on this page are recommedations for supplies used in the hydrogel embedding, ETC, and sample mounting steps of CLARITY. Most of these supplies can easily be alternated for similar supplies that perform the same task.
  +
 
==Degassing supplies==
 
==Degassing supplies==
 
===American suppliers===
 
===American suppliers===
Line 18: Line 20:
 
| Cartridge for canister to hold samples
 
| Cartridge for canister to hold samples
 
| McMaster-Carr (2204K71)
 
| McMaster-Carr (2204K71)
|-
 
| Nitrogen tank
 
| Replaces oxygen during degassing
 
| any
 
|}
 
===European suppliers===
 
{| class="wikitable"
 
|-
 
! Supply
 
! Purpose/Description
 
! Manufacturer (Part #)
 
|-
 
| Vacuum pump
 
| Degassing oxygen from container before hydrogel polymerization
 
|
 
|-
 
| Desiccation canister
 
| Canister with two outlets for nitrogen/vacuum hook-up
 
|
 
|-
 
| Desiccation cartridge
 
| Cartridge for canister to hold samples
 
|
 
 
|-
 
|-
 
| Nitrogen tank
 
| Nitrogen tank
Line 104: Line 83:
 
|}
 
|}
   
===European suppliers===
+
==ETC chamber supplies==
  +
===American suppliers===
 
{| class="wikitable"
 
{| class="wikitable"
 
|-
 
|-
Line 111: Line 90:
 
! Manufacturer (Part #)
 
! Manufacturer (Part #)
 
|-
 
|-
| Temperature controlled water circulator
+
| Plastic chamber, 60 mL
| Circulates and heats/cools clearing solution during ETC
+
| ETC container
|
+
| Nalgene (2118-0002)
 
|-
 
|-
| Buffer filter
+
| Platinum wire, 0.5 mm diameter, 40-50 cm
| Filter and canister for collecting large particle contaminants from clearing solution
+
| Wire for ETC electrodes
|
+
| Sigma Aldrich (267201-2G)
 
|-
 
|-
| Replacement filter cartridge
+
| Single barbed tube fitting, 7/16" hex head for 1/4" tubing
| Replacement filters for clearing solution filtration (replace every few ETC runs)
+
| Barbed fitting for chamber inlet and outlet
|
+
| McMaster-Carr (5463K245)
 
|-
 
|-
| BioRad HC PowerPac system
+
| Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size
| Power supply for high-current applications, 100-120/220-240 V
+
| Push-to-connect fitting for chamber inlet and outlet
|
+
| McMaster-Carr (9087K13)
 
|-
 
|-
| PowerPac system adaptor
+
| Tube to tube coupling for 3/32" x 1/16" tubing
| 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies
+
| Electrode portal for connection to power supply
|
+
| McMaster-Carr (5117K51)
 
|-
 
|-
| Patch cord for banana plugs to alligator clips, 12"
+
| 3M Duo Adhesive dispenser
| Cord to connect power supply to Pt wire of the ETC chamber
+
| Epoxy adhesive dispenser
|
+
| McMaster-Carr (7467A43)
 
|-
 
|-
| Clear 1/4" tubing
+
| 3M Duo Adhesive cartridges
| Plastic tubing for connecting ETC components with barbed fittings
+
| 50 mL epoxy adhesive cartridges
|
+
| McMaster-Carr (7467A17)
 
|-
 
|-
| Clear polyurethane tubing
+
| 3M Duo Adhesive mixing applicators
| Plastic tubing for connecting ETC components with push-to-connect fittings
+
| Cartridge tip to mix duo adhesive prior to application
|
+
| McMaster-Carr (7467A12)
 
|-
 
|-
| Chemical resistant polypropylene stopcock
+
| Cell strainer
| Stopcock with barbed fittings for connecting 1/4" tubing
+
| Tissue sample holder inside chamber
|
+
| BD Falcon (352340)
 
|-
 
|-
| Polypropylene push-to-connect ball valve
+
| Polyethylene mesh sleeve, 1/4"-1" diameter
| Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow)
+
| Mesh to place around tissue sample within holder to prevent sample movement/tumbling
|
+
| McMaster-Carr (5969K21/5969K22)
 
|-
 
|-
| Single barbed tube fitting, 9/16" hex head for 1/4" tubing
+
| Chemical resistant polypropylene mesh, 6x6, 0.1323" opening
| Barbed fitting for connecting tubing to buffer filter
+
| Mesh to place between sample holder and electrodes
|
+
| McMaster-Carr (9275T38)
  +
|-
  +
| Electronic pliers
  +
| Pliers for shaping Pt wires into electrodes
  +
| Xcelite (378M)
  +
|-
  +
| Aluminum foil tape
  +
| Tape for lightproofing sample chamber
  +
| E&K Scientific (T592100)
  +
|}
  +
==Imaging chamber supplies==
  +
===American suppliers===
  +
{| class="wikitable"
 
|-
 
|-
| Teflon tape
+
! Supply
| Wrap for sealing screwed fittings
+
! Purpose/Description
|
+
! Manufacturer (Part #)
  +
|-
  +
| WillCo-dish
  +
| PELCO black wall glass bottom dish
  +
| Ted Pella (14032)
  +
|-
  +
| BluTack
  +
| Sticky tack for holding glass dishes/plates together to form chamber
  +
| Bostik
  +
|-
  +
| Kwik-Sil
  +
| Quick-curing silicone elastomer to seal imaging chamber
  +
| World Precision Instruments (KWIK-SIL)
  +
|-
  +
| FocusClear
  +
| Refractive index matching immersion medium
  +
| CelExplorer Labs
  +
|}
  +
==Other supplies==
  +
{| class="wikitable"
  +
|-
  +
! Supply
  +
! Purpose/Description
  +
! Manufacturer (Part #)
  +
|-
  +
| 1 mm brain matrix
  +
| Brain mold with inserts for razor blades to make uniform coronal sections after embedding
  +
| ASI Instruments (RBM-2000C)
  +
|-
  +
| ThermaSeal
  +
| Adhesive film for well plates to prevent evaporation during staining
  +
| E&K Scientific (T796100)
 
|}
 
|}

Latest revision as of 00:50, 20 January 2017

Listed on this page are recommedations for supplies used in the hydrogel embedding, ETC, and sample mounting steps of CLARITY. Most of these supplies can easily be alternated for similar supplies that perform the same task.

Contents

[edit] Degassing supplies

[edit] American suppliers

Supply Purpose/Description Manufacturer (Part #)
Vacuum pump Degassing oxygen from container before hydrogel polymerization Buchi (V-700)
Desiccation canister Canister with two outlets for nitrogen/vacuum hook-up McMaster-Carr (2204K7)
Desiccation cartridge Cartridge for canister to hold samples McMaster-Carr (2204K71)
Nitrogen tank Replaces oxygen during degassing any

[edit] ETC set-up supplies

[edit] American suppliers

Supply Purpose/Description Manufacturer (Part #)
Temperature controlled water circulator Circulates and heats/cools clearing solution during ETC Lauda-Brinkmann (ECO RE 415S)
Buffer filter Filter and canister for collecting large particle contaminants from clearing solution McMaster-Carr (4448K35)
Replacement filter cartridge Replacement filters for clearing solution filtration (replace every few ETC runs) McMaster-Carr (4422K61)
BioRad HC PowerPac system Power supply for high-current applications, 100-120/220-240 V Bio-Rad (164-5052)
PowerPac system adaptor 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies Bio-Rad (164-5064)
Patch cord for banana plugs to alligator clips, 12" Cord to connect power supply to Pt wire of the ETC chamber McMaster-Carr (6927K42)
Clear 1/4" tubing Plastic tubing for connecting ETC components with barbed fittings McMaster-Carr
Clear polyurethane tubing Plastic tubing for connecting ETC components with push-to-connect fittings McMaster-Carr (5648K71)
Chemical resistant polypropylene stopcock Stopcock with barbed fittings for connecting 1/4" tubing McMaster-Carr (48285K24)
Polypropylene push-to-connect ball valve Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow) McMaster-Carr (4379K62)
Single barbed tube fitting, 9/16" hex head for 1/4" tubing Barbed fitting for connecting tubing to buffer filter McMaster-Carr (5463K247)
Teflon tape Wrap for sealing screwed fittings McMaster-Carr (7562A17)

[edit] ETC chamber supplies

[edit] American suppliers

Supply Purpose/Description Manufacturer (Part #)
Plastic chamber, 60 mL ETC container Nalgene (2118-0002)
Platinum wire, 0.5 mm diameter, 40-50 cm Wire for ETC electrodes Sigma Aldrich (267201-2G)
Single barbed tube fitting, 7/16" hex head for 1/4" tubing Barbed fitting for chamber inlet and outlet McMaster-Carr (5463K245)
Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size Push-to-connect fitting for chamber inlet and outlet McMaster-Carr (9087K13)
Tube to tube coupling for 3/32" x 1/16" tubing Electrode portal for connection to power supply McMaster-Carr (5117K51)
3M Duo Adhesive dispenser Epoxy adhesive dispenser McMaster-Carr (7467A43)
3M Duo Adhesive cartridges 50 mL epoxy adhesive cartridges McMaster-Carr (7467A17)
3M Duo Adhesive mixing applicators Cartridge tip to mix duo adhesive prior to application McMaster-Carr (7467A12)
Cell strainer Tissue sample holder inside chamber BD Falcon (352340)
Polyethylene mesh sleeve, 1/4"-1" diameter Mesh to place around tissue sample within holder to prevent sample movement/tumbling McMaster-Carr (5969K21/5969K22)
Chemical resistant polypropylene mesh, 6x6, 0.1323" opening Mesh to place between sample holder and electrodes McMaster-Carr (9275T38)
Electronic pliers Pliers for shaping Pt wires into electrodes Xcelite (378M)
Aluminum foil tape Tape for lightproofing sample chamber E&K Scientific (T592100)

[edit] Imaging chamber supplies

[edit] American suppliers

Supply Purpose/Description Manufacturer (Part #)
WillCo-dish PELCO black wall glass bottom dish Ted Pella (14032)
BluTack Sticky tack for holding glass dishes/plates together to form chamber Bostik
Kwik-Sil Quick-curing silicone elastomer to seal imaging chamber World Precision Instruments (KWIK-SIL)
FocusClear Refractive index matching immersion medium CelExplorer Labs

[edit] Other supplies

Supply Purpose/Description Manufacturer (Part #)
1 mm brain matrix Brain mold with inserts for razor blades to make uniform coronal sections after embedding ASI Instruments (RBM-2000C)
ThermaSeal Adhesive film for well plates to prevent evaporation during staining E&K Scientific (T796100)