Supplies
From CLARITY Wiki
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==ETC set-up supplies== |
==ETC set-up supplies== |
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===American suppliers=== |
===American suppliers=== |
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| Quick-curing silicone elastomer to seal imaging chamber |
| Quick-curing silicone elastomer to seal imaging chamber |
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| World Precision Instruments (KWIK-SIL) |
| World Precision Instruments (KWIK-SIL) |
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| − | | FocusClear |
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| − | | Refractive index matching immersion medium |
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| − | | CelExplorer Labs |
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| − | ! Supply |
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| − | ! Purpose/Description |
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| − | | WillCo-dish |
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| − | | PELCO black wall glass bottom dish |
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| − | | BluTack |
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| − | | Sticky tack for holding glass dishes/plates together to form chamber |
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| − | | Kwik-Sil |
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| − | | Quick-curing silicone elastomer to seal imaging chamber |
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| FocusClear |
| FocusClear |
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Latest revision as of 00:50, 20 January 2017
Listed on this page are recommedations for supplies used in the hydrogel embedding, ETC, and sample mounting steps of CLARITY. Most of these supplies can easily be alternated for similar supplies that perform the same task.
Contents |
[edit] Degassing supplies
[edit] American suppliers
| Supply | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Vacuum pump | Degassing oxygen from container before hydrogel polymerization | Buchi (V-700) |
| Desiccation canister | Canister with two outlets for nitrogen/vacuum hook-up | McMaster-Carr (2204K7) |
| Desiccation cartridge | Cartridge for canister to hold samples | McMaster-Carr (2204K71) |
| Nitrogen tank | Replaces oxygen during degassing | any |
[edit] ETC set-up supplies
[edit] American suppliers
| Supply | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Temperature controlled water circulator | Circulates and heats/cools clearing solution during ETC | Lauda-Brinkmann (ECO RE 415S) |
| Buffer filter | Filter and canister for collecting large particle contaminants from clearing solution | McMaster-Carr (4448K35) |
| Replacement filter cartridge | Replacement filters for clearing solution filtration (replace every few ETC runs) | McMaster-Carr (4422K61) |
| BioRad HC PowerPac system | Power supply for high-current applications, 100-120/220-240 V | Bio-Rad (164-5052) |
| PowerPac system adaptor | 4 mm adaptor for converting non-IEC-certified cells to fit deeper output terminals of PowerPac power supplies | Bio-Rad (164-5064) |
| Patch cord for banana plugs to alligator clips, 12" | Cord to connect power supply to Pt wire of the ETC chamber | McMaster-Carr (6927K42) |
| Clear 1/4" tubing | Plastic tubing for connecting ETC components with barbed fittings | McMaster-Carr |
| Clear polyurethane tubing | Plastic tubing for connecting ETC components with push-to-connect fittings | McMaster-Carr (5648K71) |
| Chemical resistant polypropylene stopcock | Stopcock with barbed fittings for connecting 1/4" tubing | McMaster-Carr (48285K24) |
| Polypropylene push-to-connect ball valve | Push-to-connect tubing stopcock for 3/8" OD tubing (unrestricted flow) | McMaster-Carr (4379K62) |
| Single barbed tube fitting, 9/16" hex head for 1/4" tubing | Barbed fitting for connecting tubing to buffer filter | McMaster-Carr (5463K247) |
| Teflon tape | Wrap for sealing screwed fittings | McMaster-Carr (7562A17) |
[edit] ETC chamber supplies
[edit] American suppliers
| Supply | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Plastic chamber, 60 mL | ETC container | Nalgene (2118-0002) |
| Platinum wire, 0.5 mm diameter, 40-50 cm | Wire for ETC electrodes | Sigma Aldrich (267201-2G) |
| Single barbed tube fitting, 7/16" hex head for 1/4" tubing | Barbed fitting for chamber inlet and outlet | McMaster-Carr (5463K245) |
| Polypropylene push-to-connect tube fitting for 3/8" OD, 1/4 pipe size | Push-to-connect fitting for chamber inlet and outlet | McMaster-Carr (9087K13) |
| Tube to tube coupling for 3/32" x 1/16" tubing | Electrode portal for connection to power supply | McMaster-Carr (5117K51) |
| 3M Duo Adhesive dispenser | Epoxy adhesive dispenser | McMaster-Carr (7467A43) |
| 3M Duo Adhesive cartridges | 50 mL epoxy adhesive cartridges | McMaster-Carr (7467A17) |
| 3M Duo Adhesive mixing applicators | Cartridge tip to mix duo adhesive prior to application | McMaster-Carr (7467A12) |
| Cell strainer | Tissue sample holder inside chamber | BD Falcon (352340) |
| Polyethylene mesh sleeve, 1/4"-1" diameter | Mesh to place around tissue sample within holder to prevent sample movement/tumbling | McMaster-Carr (5969K21/5969K22) |
| Chemical resistant polypropylene mesh, 6x6, 0.1323" opening | Mesh to place between sample holder and electrodes | McMaster-Carr (9275T38) |
| Electronic pliers | Pliers for shaping Pt wires into electrodes | Xcelite (378M) |
| Aluminum foil tape | Tape for lightproofing sample chamber | E&K Scientific (T592100) |
[edit] Imaging chamber supplies
[edit] American suppliers
| Supply | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| WillCo-dish | PELCO black wall glass bottom dish | Ted Pella (14032) |
| BluTack | Sticky tack for holding glass dishes/plates together to form chamber | Bostik |
| Kwik-Sil | Quick-curing silicone elastomer to seal imaging chamber | World Precision Instruments (KWIK-SIL) |
| FocusClear | Refractive index matching immersion medium | CelExplorer Labs |
[edit] Other supplies
| Supply | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| 1 mm brain matrix | Brain mold with inserts for razor blades to make uniform coronal sections after embedding | ASI Instruments (RBM-2000C) |
| ThermaSeal | Adhesive film for well plates to prevent evaporation during staining | E&K Scientific (T796100) |