Transcardial Perfusion

Transcardial perfusion of the animal using the hydrogel solution in place of normal fixative is recommended, but not essential. By adding acrylamide monomers into the tissue at the same time as paraformaldehyde, it is easier for the acrylamide to attach to the biomolecules inside the tissue. Perfusiion also quickly introduces hydrogel solution uniformly throughout the tissue so that passive diffusion of the monomers over time is not the only method of penetration.