Imaging
From CLARITY Wiki
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| − | Following [[Sample Clearing|sample clearing]] in the [[Solutions#Clearing Solution|clearing solution]], the hydrogel-embedded tissue is [[Sample Clearing#PBST buffer wash after clearing|washed in PBST buffer]] to remove SDS micelles. |
+ | Following [[Sample Clearing|sample clearing]] in the [[Solutions#Clearing Solution|clearing solution]], the hydrogel-embedded tissue is [[Sample Clearing#PBST buffer wash after clearing|washed in PBST buffer]] to remove SDS micelles. If the sample was labelled with fluorescent proteins prior to CLARITY processing (from a transgenic mouse line, for example), the proteins will be attached to the hydrogel matrix, and the sample can be imaged immediately after the PBST washing step. Otherwise, the clarified samples can be labelled via [[Immunostaining|immunostaining]] and then prepared for imaging. |
==Sample preparation== |
==Sample preparation== |
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Revision as of 19:09, 20 April 2014
Following sample clearing in the clearing solution, the hydrogel-embedded tissue is washed in PBST buffer to remove SDS micelles. If the sample was labelled with fluorescent proteins prior to CLARITY processing (from a transgenic mouse line, for example), the proteins will be attached to the hydrogel matrix, and the sample can be imaged immediately after the PBST washing step. Otherwise, the clarified samples can be labelled via immunostaining and then prepared for imaging.
Contents |
Sample preparation
Refractive index matching solutions
FocusClear
Sample mounting
Imaging techniques
Confocal microscopy
(eventually give own page)
Advantages
Disadvantages
Light sheet microscopy
(eventually give own page)