Imaging

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Following [[Sample Clearing|sample clearing]] in the [[Solutions#Clearing Solution]], the hydrogel-embedded tissue is [[Sample Clearing#PBST buffer wash after clearing|washed in PBST buffer]] to remove SDS micelles.
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Following [[Sample Clearing|sample clearing]] in the [[Solutions#Clearing Solution|clearing solution]], the hydrogel-embedded tissue is [[Sample Clearing#PBST buffer wash after clearing|washed in PBST buffer]] to remove SDS micelles.
   
 
==Sample preparation==
 
==Sample preparation==

Revision as of 19:00, 20 April 2014

Following sample clearing in the clearing solution, the hydrogel-embedded tissue is washed in PBST buffer to remove SDS micelles.

Contents

Sample preparation

Refractive index matching solutions

FocusClear

Sample mounting

Imaging techniques

Confocal microscopy

(eventually give own page)

Advantages

Disadvantages

Light sheet microscopy

(eventually give own page)

Advantages

Disadvantages

Sample storage after imaging

Image analysis