CLARITY may seem like an intimidating technique requiring a lot of time, effort, and expensive equipment to get it started and working consistently. However, many of the CLARITY steps can be simplified to make the process easier to get started.

The following recommendations are useful for getting started with CLARITY and getting a feel for performing each step and what to expect from the tissue sample in each stage. Once confident that the technique is working as it should, modifications that may increase complexity can be added. Depending on the application, the simplified CLARITY approach may be all that is needed.