Listed below are the chemicals necessary to make both the hydrogel and clearing solutions as well as suggested suppliers.
Hydrogel solution reagents
American suppliers
Reagent
|
Purpose/Description
|
Manufacturer (Part #)
|
40% acrylamide solution
|
Hydrogel monomer solution, 500 mL
|
Bio-Rad (161-0140)
|
2% bis-acrylamide solution
|
Chemical crosslinker, 500 mL
|
Bio-Rad (161-0142)
|
Formaldehyde
|
Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
|
Electron Microscopy Sciences (15710-S)
|
VA-044 photoinitiator
|
Non-nitrile azo thermal initiator with low decomposition temperature
|
Wako (VA-044)
|
10X PBS
|
Solution buffer, 500 mL
|
Invitrogen GIBCO (10010)
|
European suppliers
Reagent
|
Purpose/Description
|
Manufacturer (Part #)
|
40% acrylamide solution
|
Hydrogel monomer solution, 500 mL
|
|
2% bis-acrylamide solution
|
Chemical crosslinker, 500 mL
|
|
Formaldehyde
|
Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
|
|
VA-044 photoinitiator
|
Non-nitrile azo thermal initiator with low decomposition temperature
|
|
10X PBS
|
Solution buffer, 500 mL
|
|
Clearing solution reagents
American suppliers
Reagent
|
Purpose/Description
|
Manufacturer (Part #)
|
Boric acid
|
pH buffer
|
Sigma (B7901)
|
NaOH pellets
|
Adjust solution pH to 8.5
|
EMD (SX0590-3)
|
SDS (powder or aqueous solution)
|
Lipid-clearing detergent
|
Sigma (L3771); Amresco (0837)
|
European suppliers
Reagent
|
Purpose/Description
|
Manufacturer (Part #)
|
Boric acid
|
pH buffer
|
|
NaOH pellets
|
Adjust solution pH to 8.5
|
|
SDS (powder or aqueous solution)
|
Lipid-clearing detergent
|
|