Chemical Reagents
From CLARITY Wiki
Revision as of 07:00, 3 December 2013 by Kristin Engberg (Talk | contribs)
Contents |
Hydrogel solution reagents
American suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| 40% acrylamide solution | Hydrogel monomer solution, 500 mL | Bio-Rad (161-0140) |
| 2% bis-acrylamide solution | Chemical crosslinker, 500 mL | Bio-Rad (161-0142) |
| Formaldehyde | Biomolecule crosslinker, 16% paraformaldehyde aqueous solution | Electron Microscopy Sciences (15710-S) |
| VA-044 photoinitiator | Non-nitrile azo thermal initiator with low decomposition temperature | Wako (VA-044) |
| 10X PBS | Solution buffer, 500 mL | Invitrogen GIBCO (10010) |
European suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| 40% acrylamide solution | Hydrogel monomer solution, 500 mL | |
| 2% bis-acrylamide solution | Chemical crosslinker, 500 mL | |
| Formaldehyde | Biomolecule crosslinker, 16% paraformaldehyde aqueous solution | |
| VA-044 photoinitiator | Non-nitrile azo thermal initiator with low decomposition temperature | |
| 10X PBS | Solution buffer, 500 mL |
Clearing solution reagents
American suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Boric acid | pH buffer | Sigma (B7901) |
| NaOH pellets | Adjust solution pH to 8.5 | EMD (SX0590-3) |
| SDS (powder or aqueous solution) | Lipid-clearing detergent | Sigma (L3771); Ameresco |
European suppliers
| Reagent | Purpose/Description | Manufacturer (Part #) |
|---|---|---|
| Boric acid | pH buffer | |
| NaOH pellets | Adjust solution pH to 8.5 | |
| SDS (powder or aqueous solution) | Lipid-clearing detergent |