Chemical Reagents

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| 10X PBS
  
| 10X PBS
 
| Solution buffer, 500 mL
  
| Solution buffer, 500 mL
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|
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|}
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  +
==Clearing solution reagents==
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===American suppliers===
  +
{| class="wikitable"
  +
|-
  +
! Reagent
  +
! Purpose/Description
  +
! Manufacturer (Part #)
  +
|-
  +
| Boric acid
  +
| pH buffer
  +
| Sigma (B7901)
  +
|-
  +
| NaOH pellets
  +
| Adjust solution pH to 8.5
  +
| EMD (SX0590-3)
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|-
  +
| SDS (powder or aqueous solution)
  +
| Lipid-clearing detergent
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| Sigma (L3771); Ameresco
  +
|}
  +
  +
===European suppliers===
  +
{| class="wikitable"
  +
|-
  +
! Reagent
  +
! Purpose/Description
  +
! Manufacturer (Part #)
  +
|-
  +
| Boric acid
  +
| pH buffer
  +
|
  +
|-
  +
| NaOH pellets
  +
| Adjust solution pH to 8.5
  +
|
  +
|-
  +
| SDS (powder or aqueous solution)
  +
| Lipid-clearing detergent
 
|
 
|
 
|}
  
|}

Revision as of 07:00, 3 December 2013

Contents

Hydrogel solution reagents

American suppliers

Reagent Purpose/Description Manufacturer (Part #)
40% acrylamide solution Hydrogel monomer solution, 500 mL Bio-Rad (161-0140)
2% bis-acrylamide solution Chemical crosslinker, 500 mL Bio-Rad (161-0142)
Formaldehyde Biomolecule crosslinker, 16% paraformaldehyde aqueous solution Electron Microscopy Sciences (15710-S)
VA-044 photoinitiator Non-nitrile azo thermal initiator with low decomposition temperature Wako (VA-044)
10X PBS Solution buffer, 500 mL Invitrogen GIBCO (10010)

European suppliers

Reagent Purpose/Description Manufacturer (Part #)
40% acrylamide solution Hydrogel monomer solution, 500 mL
2% bis-acrylamide solution Chemical crosslinker, 500 mL
Formaldehyde Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
VA-044 photoinitiator Non-nitrile azo thermal initiator with low decomposition temperature
10X PBS Solution buffer, 500 mL

Clearing solution reagents

American suppliers

Reagent Purpose/Description Manufacturer (Part #)
Boric acid pH buffer Sigma (B7901)
NaOH pellets Adjust solution pH to 8.5 EMD (SX0590-3)
SDS (powder or aqueous solution) Lipid-clearing detergent Sigma (L3771); Ameresco

European suppliers

Reagent Purpose/Description Manufacturer (Part #)
Boric acid pH buffer
NaOH pellets Adjust solution pH to 8.5
SDS (powder or aqueous solution) Lipid-clearing detergent
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