Chemical Reagents

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===American suppliers===
 
===American suppliers===
 
{| class="wikitable"
 
{| class="wikitable"
  +
|-
  +
! Reagent
  +
! Purpose/Description
  +
! Manufacturer (Part #)
  +
|-
  +
| 40% acrylamide solution
  +
| Hydrogel monomer solution, 500 mL
  +
| Bio-Rad (161-0140)
  +
|-
  +
| 2% bis-acrylamide solution
  +
| Chemical crosslinker, 500 mL
  +
| Bio-Rad (161-0142)
  +
|-
  +
| Formaldehyde
  +
| Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
  +
| Electron Microscopy Sciences (15710-S)
  +
|-
  +
| VA-044 photoinitiator
  +
| Non-nitrile azo thermal initiator with low decomposition temperature
  +
| Wako (VA-044)
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|-
  +
| 10X PBS
  +
| Solution buffer, 500 mL
  +
| Invitrogen GIBCO (10010)
  +
|}
  +
  +
===European suppliers===
  +
{| class="wikitable"
  +
|-
  +
! Reagent
  +
! Purpose/Description
  +
! Manufacturer (Part #)
  +
|-
  +
| 40% acrylamide solution
  +
| Hydrogel monomer solution, 500 mL
  +
|
  +
|-
  +
| 2% bis-acrylamide solution
  +
| Chemical crosslinker, 500 mL
  +
|
  +
|-
  +
| Formaldehyde
  +
| Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
  +
|
  +
|-
  +
| VA-044 photoinitiator
  +
| Non-nitrile azo thermal initiator with low decomposition temperature
  +
|
  +
|-
  +
| 10X PBS
  +
| Solution buffer, 500 mL
  +
|
  +
|}

Revision as of 06:54, 3 December 2013

Hydrogel solution reagents

American suppliers

Reagent Purpose/Description Manufacturer (Part #)
40% acrylamide solution Hydrogel monomer solution, 500 mL Bio-Rad (161-0140)
2% bis-acrylamide solution Chemical crosslinker, 500 mL Bio-Rad (161-0142)
Formaldehyde Biomolecule crosslinker, 16% paraformaldehyde aqueous solution Electron Microscopy Sciences (15710-S)
VA-044 photoinitiator Non-nitrile azo thermal initiator with low decomposition temperature Wako (VA-044)
10X PBS Solution buffer, 500 mL Invitrogen GIBCO (10010)

European suppliers

Reagent Purpose/Description Manufacturer (Part #)
40% acrylamide solution Hydrogel monomer solution, 500 mL
2% bis-acrylamide solution Chemical crosslinker, 500 mL
Formaldehyde Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
VA-044 photoinitiator Non-nitrile azo thermal initiator with low decomposition temperature
10X PBS Solution buffer, 500 mL