Chemical Reagents

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Listed below are the chemicals necessary to make both the hydrogel and clearing solutions as well as suggested suppliers.
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==Hydrogel solution reagents==
  
==Hydrogel solution reagents==
 
===American suppliers===
  
===American suppliers===
 
{| class="wikitable"
  
{| class="wikitable"
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|-
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! Reagent
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! Purpose/Description
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! Manufacturer (Part #)
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|-
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| 40% acrylamide solution
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| Hydrogel monomer solution, 500 mL
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| Bio-Rad (161-0140)
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|-
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| 2% bis-acrylamide solution
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| Chemical crosslinker, 500 mL
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| Bio-Rad (161-0142)
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|-
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| Formaldehyde
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| Biomolecule crosslinker, 16% paraformaldehyde aqueous solution
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| Electron Microscopy Sciences (15710-S)
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|-
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| VA-044 photoinitiator
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| Non-nitrile azo thermal initiator with low decomposition temperature
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| Wako (VA-044)
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|-
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| 10X PBS
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| Solution buffer, 500 mL
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| Invitrogen GIBCO (10010)
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|}
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==Clearing solution reagents==
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===American suppliers===
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{| class="wikitable"
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|-
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! Reagent
  +
! Purpose/Description
  +
! Manufacturer (Part #)
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|-
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| Boric acid
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| pH buffer
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| Sigma (B7901)
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|-
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| NaOH pellets
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| Adjust solution pH to 8.5
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| EMD (SX0590-3)
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|-
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| SDS (powder or aqueous solution)
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| Lipid-clearing detergent
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| Sigma (L3771); Amresco (0837)
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|}

Latest revision as of 01:21, 15 December 2016

Listed below are the chemicals necessary to make both the hydrogel and clearing solutions as well as suggested suppliers.

Contents

[edit] Hydrogel solution reagents

[edit] American suppliers

Reagent Purpose/Description Manufacturer (Part #)
40% acrylamide solution Hydrogel monomer solution, 500 mL Bio-Rad (161-0140)
2% bis-acrylamide solution Chemical crosslinker, 500 mL Bio-Rad (161-0142)
Formaldehyde Biomolecule crosslinker, 16% paraformaldehyde aqueous solution Electron Microscopy Sciences (15710-S)
VA-044 photoinitiator Non-nitrile azo thermal initiator with low decomposition temperature Wako (VA-044)
10X PBS Solution buffer, 500 mL Invitrogen GIBCO (10010)

[edit] Clearing solution reagents

[edit] American suppliers

Reagent Purpose/Description Manufacturer (Part #)
Boric acid pH buffer Sigma (B7901)
NaOH pellets Adjust solution pH to 8.5 EMD (SX0590-3)
SDS (powder or aqueous solution) Lipid-clearing detergent Sigma (L3771); Amresco (0837)
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