Chamber Construction

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Chamber construction procedure

Marked chamber.jpg

Mark the plastic container

Screw down the cap of a 60 mL Nalgene container tightly. While tightly shut, mark the areas to be soldered for the inlet and outlet fittings, making sure that they are on the same plane and on opposite sides of the container. Also, mark the top and bottom of the container with some type of symbol or initials in order to be able to identify matching caps and bottoms later on (if making more than one chamber).

Solder holes for fittings

Solder holes into the marked areas of the Nalgene container. Once the hole is big enough, screw in the inlet/outlet fitting while the plastic is still hot and melted. The fitting does not need to be fully screwed in, but it should be inserted just enough such that it cannot be easily pulled out by tugging.


Add epoxy to seal

Solder holes for electrode fittings

Construct platinum wire electrodes

Add epoxy to seal

Cover with foil (if desired)

Alternative fittings

  • Push-to-connect fittings for better leakage control
  • Banana plug fittings for electrodes to prevent platinum wire breakage from alligator clips

Alternative chambers

  • 3-D printed
  • 3-D ceramic
  • 125 mL Nalgene for larger tissues